Product Description |
Recombinant Mouse GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Lys141. |
Product Background |
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
GM-CSF |
Synonyms |
CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala18-Lys141 |
Molecular Weight |
The protein has a predicted MW of 14.11 kDa same as Bis-Tris PAGE result. |
Activity |
The affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
|
Form
|
Lyophilized |
|
Shipping
|
Shipped at ambient temperature. |
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Formulation
|
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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Reconstitution
|
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
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Stability And Storage
|
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
 |
Mouse GM-CSF R alpha, His Tag captured on CM5 Chip via Anti-his antibody can bind Mouse GM-CSF, No Tag with an affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). |
 |
Mouse GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
 |
The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC. |
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