Product Description |
Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189. |
Product Background |
Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
SCF |
Synonyms |
MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu26-Ala189 |
Molecular Weight |
The protein has a predicted MW of 18.45 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.02 EU per ug by the LAL method. |
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Form
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Lyophilized |
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Shipping
|
Shipped at ambient temperature. |
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Formulation
|
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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Reconstitution
|
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
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Stability And Storage
|
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
 |
Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). |
 |
Human SCF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
 |
Measured in a cell proliferation assay using Mo7e cells. The ED50-for this effect is 1-8 ng/mL (QC Test). |
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